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Legal Resources | Code of Federal Regulations, Title 49 |    40.85  

What drugs do laboratories test for? Part 40—Procedures For Transportation Workplace Drug And Alcohol Testing Programs. Subpart F—Drug Testing Laboratories.   

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As a laboratory, you must test for the following five drugs or classes of drugs in a DOT drug test. You must not test "DOT specimens" for any other drugs.

(a) Marijuana metabolites.

(b) Cocaine metabolites.

(c) Amphetamines.

(d) Opiate metabolites.

(e) Phencyclidine (PCP).

(Authority: 49 U.S.C. 301.)